TABLE 2.
Oligonucleotide probes and primers used in this study
| Oligonucleotide | Probe | Sequence 5′-3′ |
|---|---|---|
| Probea | ||
| RPM1 | 3 | ACTTTTTATTAATATATATATATGGACTCCTGCGGGG |
| tRNAfMet | 1 | GCAATAATACGATTTGAACG |
| tRNAPro | 5 | AAGAAAGCGCCTGACCTTTTG |
| 5′ junction | 2 | ATAATATAAATATCTTATTCAAATTAAT |
| 3′ junction | 4 | AAATTAATTATGAATATGGATATTATATT |
| tRNAGlu | AGGTGATGTCGTAACCATTAGACG | |
| tRNASer | ATCACACTTTAAACCACTCAGTCAAC | |
| SCR1 | GTCTAGCCGCGAGGAAGG | |
| Primer | ||
| CEM1-5′kan | CTGTCCTCGGTGTTGCCTAATTTTAAATTAAAGATCATTTCTTTACGATGTCCACGAGGTC | |
| CEM1-3′kan | TTAAGTTTTTGCTAATATTACCCTATATATATATTCAAATTATTATAATCGGTGTCGGTCTCG | |
| HTD2-A | CTTTTAAATCATAGCCCAAA | |
| HTD2-B | CACCTTTTTTTCAGTTCTTC | |
| CBP2-A | ATAAGACAGTTCATTGTGGCA | |
| CBP2-B | AAAGGAAAGAGTGAAGATTGC | |
| HFA1-A | ATGAGATCTATAAGAAAATGGGC | |
| HFA1-B | CTATCTCTTTCGCTTACTGTCC | |
| PET127-A | TCATCTTTGAGTATATCACGTC | |
| PET127-B | CATAATATGTACCAAGGGACT | |
| PET127-up | CAGGGCACTTGAGAGAGCAC | |
| PET127-down | CCCAACGCTGACTACTGTCT |
a
Probes are antisense.