FIG. 6.

Effect of CR2 mutagenesis on wild-type gp350 binding (ELISA). (A) Alanine and proline substitutions mapped onto a ribbon representation of CR2 SCR1-2. The scheme used to color residues represents the percent binding of wild-type gp350 to mutant forms of MBP-CR2 SCR1-2 (at a concentration of 5 μg/ml gp350 and 2 μg/ml of mutant MBP-CR2 SCR1-2). The image in panel B was constructed similarly to that for panel A, except the molecule has been rotated about the y axis by 90°. N-term, N terminus; C-term, C terminus.