FIG. 4.

HCV augments the phosphorylation status of STAT3. (A) Huh-7 cells were transfected with HCV-FL, HCV-core, HCV-NS5A, or vector control and treated with 10 nM DHT. The phosphorylation status of STAT3 and total STAT3 were detected by Western blot analysis using anti-phospho-STAT3 (pSer-727 or pTyr-705) or anti-STAT3 antibody. (B) Mock- or HCV-infected IHH were treated with 10 nM DHT. The results of Western blot analysis of S⁷²⁷STAT3 and total STAT3 are shown. The expression of HCV core protein is shown from HCV-infected cell lysates by Western blot analysis using a rabbit anti-core antibody. (C) IHH were infected with HCV or left uninfected (mock). Total RNA was isolated after 72 h of infection, and RT-PCR was performed for AR mRNA expression.