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. 2008 Sep 10;82(22):11354–11361. doi: 10.1128/JVI.00956-08

FIG. 2.

FIG. 2.

(A) Single-step growth of the UL37 recombinant virus. Vero or BD45 cells (5 × 105) were infected with the indicated viruses at an MOI of 10 PFU/cell and the infected cells harvested at 2, 6, 12, and 24 h postinfection (1-ml final volume). K37eGFP2 was used in this experiment. Virus yields were determined by titration on Vero or BD45 cells. The data are averages for two experiments. (B) UL37eGFP polypeptide accumulation in infected cells. Vero cells (1 × 106) were infected with KOS and K37eGFP1/2 or mock infected (MI) at an MOI of 10 PFU/cell and the cells harvested at 8 and 24 h postinfection. The lysates prepared were analyzed by SDS-PAGE (12% acrylamide) and the proteins transferred to a membrane for Western blot analysis. Membranes were probed with anti-UL37C (αUL37C) and αGFP antibodies.