FIG. 2.
VRP-MPV.F induced hMPV-F- or hMPV-G-specific antibodies in the mucosal secretions of VRP-vaccinated mice. DBA/2 mice were vaccinated intranasally with 106 infectious units of VRP-MPV.F or VRP-MPV.G on days 0 and 14. Nasal washes (A) or BAL fluids (B) were obtained from vaccinated mice 28 days postvaccination. An MPV-F- or MPV-G-specific enzyme-linked immunosorbent assay was performed on the samples with HRP-conjugated anti-mouse IgA antibodies. The amount of binding was determined from absorbance (optical density [OD]) of HRP-substrate complexes at 450 nm.