TABLE 2.
Serum neutralizing antibody responses against various hMPV strains in immunized DBA/2 mice or cotton rats
| Immunization | Dose (log10 IU or PFU) | 60% plaque reduction serum neutralizing titer (mean log2 titer ± SD) against hMPVa in:
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| DBA/2 mice
|
Cotton rats
|
||||||||
| A1 | A2 | B1 | B2 | A1 | A2 | B1 | B2 | ||
| PBS | ≤4.3b | ≤4.3 | ≤4.3 | ≤4.3 | ≤4.3 | ≤4.3 | ≤4.3 | ≤4.3 | |
| VRP-RSV.F | 6.0 | ≤4.3 | ≤4.3 | ≤4.3 | ≤4.3 | ≤4.3 | ≤4.3 | ≤4.3 | ≤4.3 |
| VRP-MPV.F | 6.0 | 6.1 ± 1.7 | 6.6 ± 1.9 | ≤4.3 | ≤4.3 | 5.7 ± 1.2 | 6.7 ± 2.3 | ≤4.3 | ≤4.3 |
| VRP-MPV.G | 6.0 | ≤4.3 | ≤4.3 | ≤4.3 | ≤4.3 | ≤4.3 | ≤4.3 | ≤4.3 | ≤4.3 |
| MPV A2 | 5.9 | 6.3 ± 1.2 | 7.7 ± 1.3 | ≤4.3 | ≤4.3 | 6.0 ± 0.6 | 9.6 ± 0.9 | ≤4.3 | ≤4.3 |
a
The hMPV subgroup A1 strain was TN/96-12, the subgroup A2 strain was TN/94-49, the subgroup B1 strain was TN/98-242, and the subgroup B2 strain was TN/99-419.
b
The lower limit of detection was 4.3 log2 for the hMPV neutralization titer.