TABLE 5.
Cytokine mRNA expression in lungs of immunized DBA/2 mice following wild-type subgroup A2 hMPV challenge
| Immunization | Mean fold difference in cytokine gene expression vs uninfected controls (range)a
|
||||
|---|---|---|---|---|---|
| IFN-γ | IL-4 | IL-10 | IL-12 p40 | IL-13 | |
| PBS | 8.3 (4.6-17.9) | 2.2 (1.3-5.1) | 3.7 (2.6-7.0) | 9.3 (6.2-14.2) | 15.3 (7.4-46.6) |
| VRP-RSV.F | 5.4 (4.1-8.6) | 1.8 (1.4-3.2) | 3.9 (2.5-5.2) | 9.7 (3.7-16.9) | 11.6 (4.2-24.0) |
| VRP-MPV.F | 3.2 (2.2-4.9)* | 2.2 (1.1-3.7) | 4.4 (2.1-6.7) | 15.5 (10.9-23.9) | 10.8 (6.7-14.5) |
| VRP-MPV.G | 10.8 (6.3-19.5) | 1.9 (1.1-2.9) | 7.8 (4.7-10.0)* | 12.3 (6.3-19.8) | 15.8 (6.3-24.5) |
| MPV | 8.3 (4.0-10.5) | 2.1 (1.4-3.6) | 4.9 (2.3-9.3) | 14.8 (9.0-21.9) | 6.0 (3.3-13.9) |
| FI-MPV | 3.0 (2.1-6.8)* | 4.0 (2.1-8.1) | 2.9 (1.5-7.4) | 4.7 (2.3-7.5)* | 82.7 (27-208)* |
a
Values in parentheses indicate the range of fold differences between five mice in each group. *, statistically significant group difference compared to PBS-vaccinated group (P < 0.05; Mann-Whitney test).