FIG. 5.
HIV-infected human CD4 T cells cannot establish a sustained infection in cocultured rhesus CD4 cells. HIV-1SF162-infected human CD4 T cells were combined with ConA/IL-2-stimulated rhesus CD4 T cells in a 1:3 ratio. The cultures were monitored by intracellular HIV Gag staining at 3-day intervals. The left panels show the gating strategy: human and rhesus CD4 cells were distinguished by staining with human anti-CD3 (OKT3) and the pan-CD4 antibody L200. The right panels depict intracellular HIV-1 Gag levels in human and rhesus CD4+ cell populations. Data shown are representative of three independent experiments.