. 2008 Sep 3;82(22):11096–11105. doi: 10.1128/JVI.01003-08

TABLE 2.

Characterization of within-host mutations detected in individual FPV or CPV sequences^a

Virus	Virus type	Yr of isolation	nt position	Protein region	Amino acid change	Observed in nature	Functional implication of nonsynonymous change
FPV-4	FPV	1964	798	NS1	R176G	No	DNA binding^d
			809	NS1	—^b	No	—^b
			1279	NS1	A336E	No	Helicase^d
			3333	VP2	M183V	No	Unclear
FPV-3		1967	256	NS1	—^b	—^c	—^b
			339	NS1	D23N	Yes	DNA binding^d
			581	NS1	—^b	Yes	—^b
			764	NS1	—^b	Yes	—^b
			893	NS1	—^b	Yes	—^b
			1013	NS1	H247Q	Yes	DNA binding^d
			1015	NS1	T248I	Yes	DNA binding^d
			2166	NS1/NS2	V632I (NS1) R141H (NS2)	No	VP2 phosphorylation^d
			3876	VP2	A363P	No	Helicase^d
			4077	VP2	—^b	Yes	—^b
			4600	VP2	—^b	—^c	—^b
FPV-8	FPV	1989	396	NS1	V42L	No	DNA binding^d
			3088	VP2	I101T	Yes	TfR binding^f
FPV-Kai	FPV	2006	808	NS1	A179V	No	DNA binding^d
			1815	NS1	K515E	No	VP2 phosphorylation^d
			2534	VP1	—^b	Yes	—^b
			2954	VP2	—^b	No	—^b
			2957	VP2	—^b	Yes	—^b
			4578	VP2	—^b	—^c	—^b
			4760	VP2	—^b	—^c	—^b
CPV-5	CPV-2	1979	4302	VP2	—^b	No	Unclear
CPV-6	CPV-2	1980	—^b	—^b	—^b	—^b	—^b
CPV-13	CPV-2a		581	NS1	—^b	Yes	—^b
		1981	1943	NS1	—^b	No	—^b
			2090	NS1/NS2	D606E (NS1)/P116T (NS2)	No	Intracellular trafficking^d
CPV-411a^e	CPV-2b	1998	719	NS1	—^b	No	—^b
CPV-411b^e	CPV-2b	1998	3157	VP2	G124E	No	—^b
CPV-410	CPV-2b	2000	963	NS1	S231G	No	Unclear
CPV-411	CPV-2b	1998	413	NS1	—^b	Yes	—^b
		1998	941	NS1	—^b	Yes	—^b
			1319	NS1	—^b	Yes	—^b
			1481	NS1	—^b	Yes	—^b
			1562	NS1	—^b	Yes	—^b
			2195	NS1/NS2	D151N (NS2)	Yes	VP2 phosphorylation^d
			2247	VP1	—^b	Yes	—^b
			2358	VP2	—^b	Yes	—^b
			2846	VP2	—^b	Yes
			2951	VP2	—^b	Yes	—^b
			3157	VP2	G124E	No	Unclear
			3323	VP2	—^b	Yes	—^b
			3749	VP2	N321K	Yes	Possibly TfR binding
			3947	VP2	—^b	Yes	—^b
			4023	VP2	—^b	Yes	—^b
			4494	VP2	K570E	Yes	Unclear
			4510	VP2	—^b	Yes	—^b
			4526	VP2	—^b	—^c	—^b

Shown are the locations of single mutations detected in FPV or CPV samples. Nonsynonymous mutations are further characterized, and mutations present in other GenBank sequences are indicated by boldface type.

—, not applicable (synonymous change).

—, sequence coverage in GenBank was insufficient to assess the presence or absence from the population.

Functions represent those reported for the related minute virus of mice and have not been analyzed for FPV or CPV.

Since CPV-411.us.98 most likely contained two coinfecting virus strains, we analyzed the two virus populations separately and then compared the two consensus sequences present in sample CPV-411.

Transferrin receptor.