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. 2008 Sep 3;82(22):11045–11053. doi: 10.1128/JVI.01593-08

FIG. 2.

FIG. 2.

Specificity of p49 antibody and cellular localization of p49. (A) The newly raised p49 antibody was used to detect endogenous p49 by immunoblotting after 18 h treatment of MEFs or RAW 264.7 cells with 1,000 U of IFN-β/ml. (B) Immunoprecipitation of p49 was done with cell lysates of MEFs stably expressing Myc-p49 or Myc-p56. Precipitated protein was detected by Myc-immunoblotting. (C) Specificity of p49 antibody was demonstrated with cell lysates from HT1080 cells transfected with vectors for expression of Myc-p49, -p54, or -p56 or Myc-human P60, the homolog of murine p49. (D) Cellular localization of endogenous p49, induced in MEFs by 16 h of treatment with IFN-β, was examined by immunofluorescence.