TABLE 1.
Oligonucleotides used in this study
| Name or locus | Orientationa | Sequence (5′→3′) | Temp (°C)b | Time (s)c | PCR product (bp) | Efficiencyd |
|---|---|---|---|---|---|---|
| MIE-P | Fw | CTTACGGGACTTTCCTACTTG | 58 | 15 | 284 | 1.87 |
| Rv | CGATCTGACGGTTCACTAA | |||||
| MIE-T | Fw | CCTAGTGTGGATGACCTA | 58 | 8 | 112 | 1.88 |
| Rv | GTGACACCAGAGAATCAG | |||||
| UL54-P | Fw | CACCAAAGACACGTCGTT | 58 | 8 | 71 | 1.94 |
| Rv | GTCCTTTGCGACCAGAAT | |||||
| UL54-T | Fw | GTGTGCAACTACGAGGTA | 58 | 8 | 113 | 1.96 |
| Rv | GACAGCACGTTGGTTACA | |||||
| UL32-P | Fw | ACTGGTACTGCGGTTCTA | 58 | 8 | 150 | 1.88 |
| Rv | TCCACAACACCACGGTGA | |||||
| UL32-T | Fw | CCGATTACAACGACGTCA | 66-56e | 8 | 106 | 1.77 |
| Rv | GTGGATGTCGTCGTCATT | |||||
| oriLyt | Fw | GAATACAGCGATCCCTAG | 58 | 10 | 171 | 1.86 |
| Rv | GGGTTCCACCTATCTGAA | |||||
| GAPDH | Fw | CCTCACAGTTGCCATGTA | 66-56e | 8 | 71 | 1.97 |
| Rv | GATGGTACATGACAAGGTG |
a
Fw, forward primer; Rv, reverse primer.
b
Annealing temperature used for PCR.
c
Polymerization time used for PCR.
d
PCR amplification efficiency determined as described in Roche Applied Science Technical Note no. LC 13/2001.
e
“Touch-down” conditions were used.