Generation of full-length TV cDNA, TV-NV chimeric cDNA, and RNA transcripts. (A) Cloning of TV recombinant construct. The black bar represents TV first-strand cDNA, the thin arrows show primer binding sites, the white box represents TV cDNA, and the black box (or the thick gray arrow) represents the T7 promoter (T7p). (B) Generation of TV-NV chimeric cDNA. The gray box represents a portion of NV cDNA. (C) RNA transcripts of full-length TV and NV. The arrow indicates the size of RNA transcripts, based on the size of the RNA control.