FIG. 5.

Quantification of inflammatory infiltrates in skeletal muscle from RRV-infected WT and CD11b^−/− mice. Twenty-four-day-old C57BL/6J WT or CD11b^−/− mice were infected with 10³ PFU of RRV by injection in the left rear footpad. (A) At 7 dpi (n = 8) and 10 dpi (n = 3 to 7), infiltrating leukocytes were isolated from whole quadriceps muscles of WT and CD11b^−/− mice as described in Materials and Methods and the total cell number was determined. Horizontal bars indicate the means. No statistically significant differences were detected between WT mice and CD11b^−/− mice at each time point by the Kruskal-Wallis test. (B) Total numbers of F4/80⁺, CD3⁺/NK1.1⁻, and NK1.1⁺/CD3⁻ cells isolated from RRV-infected WT (filled bars) and CD11b^−/− (open bars) mice at 7 dpi. Each bar represents the arithmetic mean ± SEM of eight mice per group. *, P value of <0.05 as determined by the Mann-Whitney test. (C) Total numbers of F4/80⁺ cells isolated from skeletal muscle from RRV-infected WT (filled bars) and CD11b^−/− (open bars) mice at 10 dpi. Each bar represents the arithmetic mean ± SEM of three mice per group. Data are representative of two independent experiments.