FIG. 4.
Cell type- and miR122-dependent CPE of an adenovirus carrying miR122 target sites. (A) The indicated cell lines were infected with Ad5/3K (AdK) or Ad5/3K-122 (K122) at a MOI of 0.05. Cells were photographed 4 days later (or 6 days later in the case of Huh7 cells) to document the appearance of a CPE. Uninfected control cell cultures are shown for comparison. (B) Huh7 cells infected with Ad5/3K-122 as described in panel A following pretreatment with the miRIDIAN miR122 inhibitor. +, present; −, absent.