FIG. 4.

Polysome distribution of UL84, IE2 86, UL44, and G6PD RNAs in the WT and IE2 86ΔSX (ΔSX) infections. (A) Absorbance measured during fractionation from the heaviest to the lightest fractions. Fractions were pooled into six separate samples (fractions 1 to 6) and are represented in the figure. Absorbance (A₂₅₄) was measured throughout the collection. The polysomal fractions are represented in fractions 1, 2, and 3. The monosomal fractions are represented in fractions 4 and 5. Fraction 6 represents the free RNP RNA. (B) The distribution of the RNA and quantification of each fraction is shown for UL84, IE2 86, UL44, and G6PD. Samples were either treated with EDTA (+EDTA) or mock treated (-EDTA). Quantified RNA was measured by real-time RT-PCR and then normalized to the total amount of RNA collected in that fraction. Relative amounts of RNA are shown compared to the total WT RNA present in fraction 6 for each gene, which was set to a value of 1.