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. 2008 Sep 15;28(22):6731–6745. doi: 10.1128/MCB.02103-07

FIG. 7.

FIG. 7.

Allelic expression of the murine 91H RNA. (A) Map of H19 region indicating positions of PCR primer pairs used in real-time PCR (mC1 and mC2; mF1 and mF2). (B) RT using a H19-sense-intron 4 primer was performed on total RNA extracted from 5-day-old mouse liver issued from SDP711XM.m.dom. (left panel) or M.m.dom.XSDP711 hybrid mice (right panel). A 301-bp PCR fragment was then obtained by using the mC primer pair and digested (D) or undigested (ND) with BglI at an M. spretus-specific restriction site. (C) Reverse transcription using an mF1 sense primer was performed on the samples described above. A 344-bp PCR fragment was then obtained using the mF primer pair and cut by XbaI at a M. musculus domesticus-specific restriction site.