TABLE 1.

Cellular localization of human 91H^a

Gene	Nuclear cell extract	Cytoplasmic extract
Rplp0	20.44	79.56
Gapdh	21.4	78.6
U3 snoRNA	73.6	26.4
H19	44.4	55.6
91H (G primers)	81.82	18.18
91H (H primers)	80.12	19.88
MrpL23	20.83	79.17

^aRelative RNA levels were determined by quantitative RT-PCR after separation of the nuclear and cytoplasmic compartments. MrpL23, Rplp0, Gapdh, and H19 RNAs were used as cytoplasmic control transcripts, and U3 snoRNA was used as a nuclear control RNA. 91H was quantified independently at the G and H regions (see Fig. 1). H19 and MrpL23 were detected using primers designed over exon/exon junctions. Values in boldface indicate the preferential localization of the transcript.