FIG. 2.

Dynamics of biofilm formation by S. pneumoniae TIGR4. Wild-type (filled bars) and acapsular mutant (open bars) bacteria were grown planktonically (A) and as biofilm (B). The corresponding CFU were determined at the indicated time points. (C) The total biofilm attached (adhered bacteria plus extracellular material) was estimated by staining with CV and measuring the absorbance at 570 nm (OD570). Bacteria were grown in 12-well polystyrene plates in THY plus 0.3% glucose in a 5% CO₂ incubator at 37°C. Results are representative of three experiments. Error bars show standard deviations.