TABLE 2.
Enumeration of infected host cells and associated C. jejuni bacteria (due to adherence and invasion) per cell after 30 min of infectiona
Strain | % of host cells with associated bacteria | No. of internalized bacteria/infected cell (range) | % of infected cells with 1 associated bacterium | % of infected cells with 2 associated bacteria | % of infected cells with 3 to 5 associated bacteria |
---|---|---|---|---|---|
Undifferentiated 1-day-old INT407 cells | |||||
81-176 | 28.02 ± 3.48 | 1-3 | 77 | 20 | 3 |
NCTC11168 | 13.36 ± 1.04 | 1-3 | 81 | 17 | 2 |
RY213 | 1.34 ± 0.30 | ≥1 | 100 | 0 | 0 |
Undifferentiated 1-day-old Caco-2 cells | |||||
81-176 | 19.76 ± 1.87 | 1-10 | 50 | 20 | 30 |
NCTC11168 | 11.56 ± 0.81 | 1-7 | 62 | 20 | 18 |
RY213 | 1.94 ± 0.39 | 1-3 | 77 | 12 | 10 |
Differentiated 7-day-old Caco-2 cells | |||||
81-176 | 11.39 ± 0.69 | 1-5 | 59 | 23 | 18 |
NCTC11168 | 16.82 ± 1.38 | 1-5 | 56 | 22 | 21 |
RY213 | 0.92 ± 0.18 | ≥1 | 100 | 0 | 0 |
Adherence assays were performed using an MOI of ∼10. Caco-2 and INT407 cells were cultured for 1 to 7 days as described in Materials and Methods.