FIG. 1.

Identification of a novel dense granule protein, GRA14. (A) Schematic of gene model 49.m03169, which depicts a predicted signal peptide (SP) and transmembrane domain (TM) within the protein's primary sequence. Also indicated are the residues in the N-terminal region used to generate a His₆ recombinant protein for polyconal antiserum production. (B) 49.m03169 antiserum detects a band in Toxoplasma cell lysates at 47 kDa by Western blot analysis. (C) IFA with 49.m03169 antiserum showing strong immunoreactivity in the PV (arrow) and colocalization with GRA3, indicating that 49.m03169 is a novel dense granule protein (GRA14). (D) IFA of infected cells fixed with acetone showing GRA14 on the rim of the PVM (double arrow) and in the PV (single arrow), indicating that GRA14 partially traffics to the PVM.