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. 2008 Sep 2;76(11):4865–4875. doi: 10.1128/IAI.00782-08

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Copyright © 2008, American Society for Microbiology

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FIG. 4. — GRA14 is detected on PVM extensions connecting neighboring vacuoles and colocalizes with PVM-associating GRA proteins. (A) Cells were infected with GRA14-HA parasites for 24 h and then analyzed by IFA. GRA14-HA immunoreactivty was found on PVM extensions that connected neighboring vacuoles. (B) GRA14-HA colocalizes with GRA7 on PVM extensions but does not appear to perfectly overlap, as distinct GRA14 and GRA7 puncta were observed (boxed area). (C) GRA14-HA-positive PVM extensions also colocalized with GRA3 and, like GRA7, did not perfectly overlap with GRA14 (boxed area). (D) GRA14-HA is not detected in evacuoles generated by cytochalasin D-arrested parasites. Evacuoles are seen with ROP7 antibody staining (positive control), but not with GRA14-HA staining.