FIG. 5.

GRA14 is transferred to neighboring vacuoles. (A) Cartoon depicting two possible fates of GRA14 in a coinfection with wild-type and Δgra14/GFP⁺ parasites. (Top) A situation where GRA14 is not transferred to neighboring Δgra14/GFP⁺ vacuoles. (Bottom) Intervacuolar GRA14 transfer by either PVM extensions or another mechanism. (B) GRA14 signal (red) was seen in neighboring vacuoles formed by Δgra14/GFP⁺ parasites (single arrows), where intervacuolar transfer occurred in ∼5 to 10% of Δgra14/GFP⁺ vacuoles. Vacuoles formed by Δgra14/GFP⁺ parasites that were not in direct contact with wild-type vacuoles did not show intervacuolar protein transport (double arrows).