FIG. 6.
Effect of Stx1 on CD77 expression by bovine colonic crypt mesenchymal cell line clone 12. Cells were incubated for 72 h with Stx1 (200 CD50/ml, as determined with Vero cells) without or after preincubation with anti-StxB1 (1.5 μg/ml). CD77 was quantified by flow cytometry after labeling of native cells (left) and of permeabilized cells (right). Student's t test detected P ≤ 0.05 (*), P ≤ 0.01 (**), and P ≤ 0.001 (***).