FIG. 7.

Effect of Stx1 on the relative quantities of gene transcripts harbored by bovine colonic crypt cell line clone 12. Cells were incubated for 24 h with Stx1 (200 CD₅₀/ml, as determined with Vero cells) without or after preincubation with anti-StxB1 (1.5 μg/ml). Subsequently, mRNA was quantified as described in the legend to Fig. 3. Data are the means of three independent experiments without (A) or with (B) additional supplementation of the culture medium with LPS. Minima and maxima are indicated by asterisks at the end of the vertical lines. In the absence of LPS, the IL-10 and IL-12 mRNA was detectable only in one of three cultures. Student's t test detected P ≤ 0.05 (*), P ≤ 0.01 (**), and P ≤ 0.001 (***). Bold asterisks above horizontal brackets indicate significant differences between Stx1- and Stx1 + anti-StxB1-treated cultures.