TABLE 1.
M. pneumoniae genes and their oligonucleotide primers used for transcriptional analysis by RT-PCR
| Gene | Primer namea | Primer sequence | Amplicon length (bp) |
|---|---|---|---|
| mpn529 | 529RTPCRP | ACAACATCGAGTAAGCCATTGTC | 317 |
| 529RTPCRM | TAGTCTGCGTATTTTCAGCGTACC | ||
| mpn530 | 530RTPCRP | GGAACCAAATAATTTAAAAGAAGAGCTAG | 404 |
| 530RTPCRM | TCGTTTAAGTAGTCAGCTATTTTTTCAG | ||
| clpB | 531RTPCRP1 | AATTTATGAACTTTCGTTGTCTGG | 426 |
| 531RTPCRM2 | CATTTCTACAGCAGCTACCAAGGC | ||
| 531RTPCRP2 | TTTACTAACTGAAGCAGTCCGACG | 544 | |
| 531RTPCRM2 | TCTCAACTATTACGTTGTAACTAACATC | ||
| licA | 532RTPCRP | TAGCGCGTAAAGATGTACCGTT | 557 |
| 532RTPCRM | CAGCAACACGTAAATTACCGTTTC | ||
| ackA | 533RTPCRP | GATTCGGTGATCGTAGATGCA | 558 |
| 533RTPCRM | AGCGAAAACATCACGCATATCC | ||
| mpn534 | 534RTPCRP | GAATACTTTTAAAGAAACCCTCTTTTCA | 408 |
| 534RTPCRM | ACCTTTCTAATTTGCTGTTTGG |
a
P, reverse primer; M, forward primer.