FIG. 1.

Infection of THP-1 macrophages with EDL933. (A) Quantification of intracellular bacteria at different times postinfection, where 0 h corresponds to 20 min of phagocytosis. (B and C) Fluorescence microscopy of infected macrophages, with EDL933-GFP and human macrophages at 2 h (B) or 24 h (C) postinfection (magnification, ×1,000). (D) Cytometry analysis of EDL933-GFP in contact with human macrophages at 2 h (gray) and 24 h (black) postinfection. All assays were conducted in duplicate and repeated independently at least three times. Results are expressed as means ± standard deviations for the replicate experiments, and the Student t test was used for statistical analysis of the data.