FIG. 3.

HA-tagged BopE is secreted by wild-type but not bsaZ mutant B. thailandensis. Wild-type (700388), bsaZ mutant (AH174), and BopE-HA-expressing wild-type (AH191) and bsaZ mutant (AH194) B. thailandensis bacteria were grown to mid-logarithmic phase in LB broth at pH 4.5, and the proteins from the supernatants were precipitated with trichloroacetic acid. BopE-HA from the precipitates (Sup) and the cell lysates (Pellet) was detected by immunoblotting with anti-HA antibody. Data are representative of the results of one of two independent experiments.