FIG. 2.

Protein profiles of strain B31 MI and the WT and isogenic ospAB clones; detection of OspA, OspB, and FlaB. (A) Coomassie blue gel shows whole-cell lysates from 35°C cultures of B31 MI, WT, ospA⁺B1, and ospA1 strains separated by SDS-PAGE and stained with Coomassie blue. (B to E) Immunoblots of replicate SDS-PAGE separations reacted as described in Materials and Methods with OspA-specific monoclonal antibody H5332 (B), OspB-specific monoclonal antibody H6831 (C), OspB-specific monoclonal antibody H4610 (D), and FlaB-specific rabbit polyclonal antiserum J5750 (E). Migration of molecular mass standards in kDa is indicated at left. αOspA, anti-OspA.