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Copies of flaB DNA in mouse tissue
| Clone | Mouse | Mean no. ± SD of copies of flaB/100 ng DNAa |
|---|---|---|
| WT | 1 | 107.1 ± 15.5 |
| 2 | 235.7 ± 12.0 | |
| 3 | 4.9 ± 2.1 | |
| ospA1 | 1 | 9.9 ± 3.0 |
| 2 | 265.4 ± 39.6 | |
| 3 | 12.1 ± 4.8 |
Quantitative PCR was performed with triplicate 100-ng aliquots of mouse ear DNA for each sample to determine the mean number of copies of flaB DNA ± standard deviation (SD) relative to that of the B31 MI DNA standard, as described in Materials and Methods.
