FIG. 6.

Dynamin-2 and β-actin are required for targeting of LKT to mitochondria. (A) Representative immunoblot of cell lysates from BL-3 cells transfected with dynamin-2 siRNA, untreated cells (control [Con cells]), or cells transfected with scrambled siRNA (Con siRNA). (B) BL-3 cells were transfected with dynamin-2 siRNA or scrambled siRNA or were not treated (Con Cells) before incubation with LKT (0.5 U) at 37°C for 1 h. Mitochondria were purified and lysed, and the lysates were immunoblotted for LKT and porin-2 (mitochondrial marker). (C) BL-3 cells were pretreated with cytochalasin D (CytD) (2 μg/ml for 1 h at 37°C) before they were treated with LKT. Mitochondria were then isolated and immunoblotted for LKT and porin-2. The controls included cells treated with cytochalasin D alone and untreated BL-3 cells.