FIG. 1.

Molecular schematic of the PyP140/RON4 gene and deduced protein. (A) pyp140/ron4 gene schematic, including two introns (I) that have been confirmed by analysis of cDNA and genomic DNA products and a λ-Zap cDNA clone (F8.4). (B) ORF (908 residues) for the deduced full-length protein, including the signal sequence (SS; blue), the locations of the three types of repetitive regions (red, orange, and green), the positions of eight cysteine residues (*), and the location of the epitope recognized by MAb 48F8 (▾). Shown in purple is the ORF from exon 3. The solid line positioned at amino acid 113 identifies the first amino acid deduced from the ORF of the F8.4 phage clone. (C) Cysteine-rich region of PyP140/RON4 expressed in P. pastoris. The solid black region at the carboxyl end of PpPyP140/RON4 represents the His₆ affinity tag. (D) Deduced amino acid sequence of Py140/RON4, with the different repeats shown in red, orange, and green and the region expressed in P. pastoris shown in bold.