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. 2008 Aug 18;76(11):4876–4882. doi: 10.1128/IAI.01717-07

FIG. 3.

FIG. 3.

Evaluation of PpPyP140/RON4-specific mouse antiserum. (A) Immunoblot. Pooled anti-PpPyP140/RON4 specific mouse serum (mαPyRON4) was blotted against purified native PyP140/RON4 protein under reducing (R) and nonreducing (NR) conditions. MAb 48F8 was included as a positive control, and appropriate negative controls are shown (secondary antibody alone [2° only] or pooled sera from mice immunized with PBS alone [m_control]). (B) IFA of PyP140/RON4 location in mature schizont-infected erythrocytes stained with MAb 48F8, antisera from mice immunized with PpPyP140/RON4, or control mouse serum (m_control).