FIG. 7.

Low dietary protein and antioxidants reduce day 3 lung lymphocytes, IFN-γ-producing cells, and DTH responses to C. pneumoniae in B6 mice. B6 mice on LL or HL diets and A/J mice on LL diet were rechallenged with C. pneumoniae and injected into the paws with C. pneumoniae antigen 2 days later. After 24 h, the DTH response was determined as an increase in paw thickness (mm⁻²) over PBS-injected paws. Lung cells from sacrificed mice were obtained by collagenase digestion for the quantification of lung lymphocytes (×200,000), and IFN-γ-producing cells (per 10⁶ lung lymphocytes) were quantified by ELISPOT assay. Asterisks indicate significant differences between B6-LL and B6-HL or A/J-LL mice (P < 0.05 by Tukey HSD test). Error bar, ±95% CI (n = 10 mice/group).