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. 1992 Aug;60(8):3446–3447. doi: 10.1128/iai.60.8.3446-3447.1992

Mouse neutrophils lack defensins.

P B Eisenhauer 1, R I Lehrer 1
PMCID: PMC257335  PMID: 1639513

Abstract

Defensins are broad-spectrum antimicrobial peptides that are abundant in human, rat, and rabbit neutrophils. We now report that neutrophils from nine strains of mice lacked appreciable defensin content. Mice may therefore be imperfect experimental surrogates for humans or rats in models of infection in which neutrophil function is significant.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Daher K. A., Lehrer R. I., Ganz T., Kronenberg M. Isolation and characterization of human defensin cDNA clones. Proc Natl Acad Sci U S A. 1988 Oct;85(19):7327–7331. doi: 10.1073/pnas.85.19.7327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Eisenhauer P. B., Harwig S. S., Szklarek D., Ganz T., Selsted M. E., Lehrer R. I. Purification and antimicrobial properties of three defensins from rat neutrophils. Infect Immun. 1989 Jul;57(7):2021–2027. doi: 10.1128/iai.57.7.2021-2027.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Eisenhauer P., Harwig S. S., Szklarek D., Ganz T., Lehrer R. I. Polymorphic expression of defensins in neutrophils from outbred rats. Infect Immun. 1990 Dec;58(12):3899–3902. doi: 10.1128/iai.58.12.3899-3902.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ganz T., Rayner J. R., Valore E. V., Tumolo A., Talmadge K., Fuller F. The structure of the rabbit macrophage defensin genes and their organ-specific expression. J Immunol. 1989 Aug 15;143(4):1358–1365. [PubMed] [Google Scholar]
  5. Ganz T., Selsted M. E., Lehrer R. I. Defensins. Eur J Haematol. 1990 Jan;44(1):1–8. doi: 10.1111/j.1600-0609.1990.tb00339.x. [DOI] [PubMed] [Google Scholar]
  6. Lehrer R. I., Ganz T. Antimicrobial polypeptides of human neutrophils. Blood. 1990 Dec 1;76(11):2169–2181. [PubMed] [Google Scholar]
  7. Ouellette A. J., Greco R. M., James M., Frederick D., Naftilan J., Fallon J. T. Developmental regulation of cryptdin, a corticostatin/defensin precursor mRNA in mouse small intestinal crypt epithelium. J Cell Biol. 1989 May;108(5):1687–1695. doi: 10.1083/jcb.108.5.1687. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ouellette A. J., Lualdi J. C. A novel mouse gene family coding for cationic, cysteine-rich peptides. Regulation in small intestine and cells of myeloid origin. J Biol Chem. 1990 Jun 15;265(17):9831–9837. [PubMed] [Google Scholar]
  9. Selsted M. E., Harwig S. S. Purification, primary structure, and antimicrobial activities of a guinea pig neutrophil defensin. Infect Immun. 1987 Sep;55(9):2281–2286. doi: 10.1128/iai.55.9.2281-2286.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Selsted M. E., Szklarek D., Lehrer R. I. Purification and antibacterial activity of antimicrobial peptides of rabbit granulocytes. Infect Immun. 1984 Jul;45(1):150–154. doi: 10.1128/iai.45.1.150-154.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Valore E. V., Ganz T. Posttranslational processing of defensins in immature human myeloid cells. Blood. 1992 Mar 15;79(6):1538–1544. [PubMed] [Google Scholar]
  12. Wray W., Boulikas T., Wray V. P., Hancock R. Silver staining of proteins in polyacrylamide gels. Anal Biochem. 1981 Nov 15;118(1):197–203. doi: 10.1016/0003-2697(81)90179-2. [DOI] [PubMed] [Google Scholar]

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