FIG. 6.

Translocation and subcellular localization of SPI2 effector proteins in BM-DC. BM-DC from C57BL/6 mice were infected at an MOI of 10 with Salmonella WT harboring plasmids for the expression of genes encoding various epitope-tagged effector protein. A mock-infected cell is shown as control. A subset of the effectors was tagged with the M45 epitope (SifA, PipB, PipB2, and SseJ); the other subset contained effectors fused to the hemagglutinin tag (SseF, SseG, SseI, SseJ, SspH1, and SspH2). At 16 h after infection, cells were fixed and processed for immunofluorescence of Salmonella LPS (green), the tag of the effector protein (red), and the CD11c (blue). Representative infected cells are shown for the specific effector proteins. Micrographs were acquired with a Zeiss Axiovert wide-field microscope equipped with an Apotome and show single Z-sections. Scale bars, 5 μm.