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Plasmids
| Plasmid | Characteristics | Source or reference |
|---|---|---|
| pKD3 | oriRg blaM; Cmr cassette flanked by FRT sites | 6 |
| pKD4 | oriRg blaM; Kanr cassette flanked by FRT sites | 6 |
| pKD46 | ori101 repA101(ts) araBp-gam-bet-exo blaM | 6 |
| pCP20 | FLP synthesis under thermal control | 3 |
| pET28-a | His6 tag expression vector | Novagen |
| pET:nleH1 | Derivative of pET28-a expressing NleH1-His6 | This study |
| pET:nleH1K159A | Derivative of pET28-a expressing NleH1K159A-His6 | This study |
