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. 2008 Sep 8;76(11):4989–4998. doi: 10.1128/IAI.00667-08

FIG. 2.

FIG. 2.

(A) Schematic of the mam (top) and mia-mam (bottom) genes showing the locations of primers used for RT-PCR. mam-F, primer mam-RT-F; R, primer mia-mam-RT-R; mia-F, mia-RT-F; E, EcoRI restriction site used for combining the mia promoter with the mam coding region. (B) Agarose gels of RT-PCR and regular PCR products obtained from amplification of the mam (91-bp product) and mia-mam (117-bp product) genes using the three primers mia-mam-RT-R, mam-RT-F, and mia-RT-F mixed together. (Top) The template was genomic DNA from wild-type strain 158 (WT) or strain Over1. (Bottom) The template was Over1 genomic DNA (Over1 PCR), Over1 RNA (Over1 RT), Over2 RNA (Over2 RT), or Over3 RNA (Over3 RT).