TABLE 2.
Oligonucleotides used in this study
| Oligonucleotide | Sequencea |
|---|---|
| TetM-F-Bam | GGTTGTGGATCCTTGTGGGTACTTTTAGGGC |
| TetM-R-Bam | CTTATTGGATCCGAAACCATATTTATATAACAAC |
| iPCR-F | CTGATTCTGTGGATAACCGTATTACCGC |
| iPCR-R | CAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTA |
| iPCR-S | TTTGAGTGAGCTGATACCGCTCGC |
| mia-PF-Not | CGGCGGCCGCAACCTTTGGAGATTGCTTC |
| mia-PR-Eco | CATTATTTTATTGTGAATTCCTTTTC |
| mam-F-Eco | CACAAATTTAAAAATCATAAGGAATTCAAAAATGAAAAC |
| mam-R-Not | CGGCGGCCGCCCCCAACCTTTTAGATTG |
| mia-RT-F | AAATTTATTAAAACAGGTAACTCCG |
| mam-RT-F | GCTGTGAAAATGAAATTCTTCAC |
| mia-mam-RT-R | GCTAAAGTGACGGTTGCG |
| Tn-F | TTTAGCAAATCATATATAAAAGCGCG |
| Tn-R | TTTTAAATAGTTTCTTTGTTTACCCGC |
| Over1-F | ACTACCGAAGGCGAAACCGC |
| Over1-R | ACATCTTCGCAAGAAGCCGC |
a
Underlined nucleotides are the nucleotides in restriction sites used for cloning of PCR products.