FIG. 8.

Active and passive immunization of mice with BD. (A) Groups of eight BALB/c mice were immunized with BD or injected with alum adjuvant as a control. The immunized mice were infected with KIM5caf1A, and mouse survival was recorded for 21 days. The difference in the survival curves was significant (BD versus alum, P < 0.05) as determined by a log rank test. (B) Naïve BALB/c mice (n = 7 per group) were infected i.v. with KIM5caf1A. Passive immunization was performed by i.p. injection of pooled alum control sera or BD sera (100 μl in each dose) on days −1, 0, and +1. BD serum was exposed to LcrV-coated beads to deplete anti-LcrV antibody prior to use in passive immunization. Alum serum was mock depleted in parallel as a control. Mouse survival was recorded for 21 days. The difference in the survival curves was significant (BD depleted versus alum depleted, P < 0.05) as determined by a log rank test.