FIG. 1.

Selective permeabilization of the eukaryotic cell membrane by PFO treatment. C. trachomatis-infected L929 cells were treated with buffer alone (A and B) or with buffer containing PFO (C and D). After treatment, monolayers were incubated in the presence of 10-kDa fluorescein-conjugated dextran (green). Chlamydiae were detected using mouse anti-MOMP antisera and a secondary anti-mouse antibody conjugated to Texas Red (A and C). Monolayers were stained with DAPI (4′,6′-diamidino-2-phenylindole) (blue) to locate cellular nuclei (B and D).