FIG. 2.

Listeriae trapped within the phagosome were not sensitive to cytosolic gentamicin. J774 macrophage-like cells were infected with wild-type (□) or hemolysin-deficient (Hly⁻) (▪) L. monocytogenes. Monolayers were subjected to one of four treatments: no treatment, PFO permeabilization only, antibiotic treatment only, or PFO permeabilization and antibiotic treatment. CFU were quantified after treatment. Each treatment was performed in duplicate, and the entire experiment was repeated three times. Error bars represent the standard error of the mean.