FIG. 5.

Developmental stage-specific localization of chlamydial proteins in the host cell cytosol. Autoradiogram of the soluble fraction obtained from C. trachomatis-infected HeLa cells after treatment with PFO at 20 hpi (A), 30 hpi (B), or 40 hpi (C). Monolayers were metabolically labeled with [³⁵S]methionine-cysteine in the presence of eukaryotic protein synthesis inhibitors. After PFO permeabilization, supernatants were precipitated with methanol and chloroform. Three sections (I to III) from each of the two-dimensional autoradiograms were enlarged to compare constellations of spots obtained at each time point: panel D, 20 hpi; panel E, 30 hpi; and panel F, 40 hpi. Open arrows indicate proteins whose abundance remains unchanged throughout the developmental cycle, and arrowheads indicate proteins that decrease throughout the course of the developmental cycle. Proteins that appear de novo (thin arrows) or increase over time (asterisks) are also indicated.