FIG. 5.
Adherence of E. coli DH5α strains to HEp-2 cells. (A) DH5α(pAT153) (nonadherent vector control). (B) DH5α(pEH32) expressing adcA of C. rodentium showing a diffuse pattern of adherence. (C) DH5α(pEH6, pEH32) expressing both adcA and regA of C. rodentium showing an enhanced diffuse adherence pattern. Bar = 50 μm. The arrows indicate adherent bacteria. The preparations were stained with Giemsa stain. The mean ± standard deviation number of adherent bacteria per HEp-2 cell is shown for each strain.