TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference(s) or source |
|---|---|---|
| Strains | ||
| DH5α | Nonpathogenic E. coli K-12 laboratory strain | 39 |
| ICC169 | Spontaneous Nalr derivative of wild-type C. rodentium biotype 4280 (Nalr) | 30, 52 |
| P1A2 | Random transposon mutant of ICC169 (Kanr) | 21, 30 |
| EMH1 | C. rodentium regA::aphA-2 (Nalr Kanr) | This study |
| EMH2 | C. rodentium adcA::aphA-2 (Nalr Kanr) | This study |
| EMH3 | C. rodentium kfc::cat (Nalr Cmr) | This study |
| Plasmids | ||
| pACYC184 | Medium-copy-number cloning vector (Cmr Tetr) | Fermentas, Burlington, Ontario, Canada |
| pAT153 | Medium-copy-number cloning vector (Ampr) | 49 |
| pBAC1 | pBluescript II carrying the cat gene from pACYC184 (Ampr Cmr) | Praszkiera |
| pEH4 | Derivative of pAT153 carrying regA (Ampr) | This study |
| pEH6 | Derivative of pACYC184 carrying regA (Cmr) | This study |
| pEH32 | Derivative of pAT153 carrying adcA (Ampr) | This study |
| pGEM-T Easy | High-copy-number cloning vector (Ampr) | Promega, Madison, WI |
| pKD46 | Red recombinase system expression plasmid (Ampr) | 9 |
| pMU2385 | Low-copy-number transcriptional fusion vector derived from pMU575 (Tmpr) | 37 |
| pTOPO-TA | Medium-copy-number cloning vector (Ampr Kanr) | Invitrogen, Carlsbad, CA |
| pUC4-KIXX | pUC4K derivative containing aphA-2 gene (kanamycin resistance cassette) from Tn5 (Kanr Bler) | Pfizer Ltd., West Ryde, NSW, Australia |
a
J. Praszkier, Department of Microbiology and Immunology, The University of Melbourne, Victoria, Australia.