TABLE 2.
Oligonucleotide primers used in this study
| Primer | Sequence (5′-3′) | Specificity |
|---|---|---|
| aidAF | CGGGATCCTAATGTCGGAGACGTCCAGG | adcA promoter region |
| aidAR | CCAAGCTTTTGTGCGAAATCGCGATAGG | adcA promoter region |
| em31 | TCTGCATGACCATATGGGATGGAGCAAGCG | regA |
| em32 | GGAATACTAAGCTTATTTCCATTAGTAGCTCCGG | regA |
| em33 | ATGGAAATAAGCTTAGTATTCCTTGAGGCCTCGG | regA |
| em34 | TTCACGAAATTCATTAGATTCATATGGCCG | regA |
| em41 | GGGGTACCTAACTTTACACCACGGACGG | regA |
| em42 | GCTGTAGAAACGCTATTTAATCCTCCGG | regA |
| em52 | TCAAGCTGATAGTTGCTGGG | adcA |
| em53 | ATCGGTTAAAGCTTAACTAAAATCAGCCATGGG | adcA |
| em54 | TTTTAGTTAAGCTTTAACCGATTTGCCTGGCAG | adcA |
| em55 | AATATCGTAATGACCGAAGG | adcA |
| em64 | CGGGATCCTGTTACCTACATCTGAGGCG | kfcC promoter region |
| em65 | CCAAGCTTTTCCTAAGTCAACACTGGCG | kfcC promoter region |
| em66 | CGAGCTCTGATAGATTACAGGTCGCAG | adcA |
| em67 | GCTCTAGATCTGATCTGTAAGCAAGCG | adcA |
| em69 | AATTGTGGCAGTCTTGGGTG | kfcC |
| em70 | CGGGATCCAATAGCCTTATCCGTCTCGG | kfcC |
| em76 | CGGGATCCAATGCCAAGTTAATAACGGG | kfcH |
| em77 | TAAATGTCACCGGCAAGCTG | kfcH |