TABLE 5.
Genes strongly activated by RegA identified by microarray analysis
| ORF(s)a | Increase (fold)b
|
Product | |
|---|---|---|---|
| LB medium | LB medium + bicarbonate | ||
| ROD3421 | 9 | 126 | Homolog of Aap (dispersin) of EAEC and virulence factor CexE of ETEC (accession no. 2JVU_A and ABM92275) |
| ROD3431, ROD3451, ROD3461, ROD3471, ROD3481 | 2 | 12 | Homolog of the Aat ABC transporter of EAEC (accession no. AY351860) |
| ROD15971 | 1 | 5 | 65% identity and 83% similarity to unknown protein of E. coli O157:H7 (accession no. NP_287725) |
| ROD16181 | 1 | 11 | 69% identity and 80% similarity to unknown protein encoded by prophage CP-933K E. coli O157:H7 (accession no. AAG55113) |
| ROD16201 | 5 | 23 | Homolog of porin protein SfpA of Y. enterocolitica (accession no. ABF0643) |
| ROD41031, ROD41041, ROD41051 | 1 | 8 | Gene cluster encoding an unknown protein and homologs of the HlyD and HlyB secretion proteins of Shewanella woodyi (accession no. ACA88067 and ACA88066) |
| ROD41241, ROD41261, ROD41271, ROD41291 | 1 | 16 | Kfcc |
| ROD41301 | 1 | 20 | AdcAc |
| ROD41311 | 13 | 56 | Unknown ORF located immediately upstream of regA |
| ROR50001 | 1 | 5 | Homolog of a putative virulence-related PagC-like membrane protein of E. coli O157:H7 (accession no. NP_289546) |
a
ORF designations were obtained from http://www.sanger.ac.uk/Projects/C_rodentium/C_rod_genome_CDS.tab.
b
The increases were derived from the average log2 ratio of the transcript levels for RegA+ strain EMH1(pEH6) to the transcript levels for the RegA− strain in the absence or presence of 45 mM bicarbonate (a value of 1 indicates no change).
c
Determined in this study.