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Expression of ses and set. (A) Detection of ses and set mRNAs by RT-PCR. S. aureus Fukuoka 5 was cultured at 37°C and collected for RNA isolation at 0 to 7 h after inoculation. The Fukuoka 5 growth curve was graphed by determining the optical density at 600 nm (O.D.600). (B) Detection of SES and SET in culture supernatants of S. aureus strains harboring plasmid pF5 (Fukuoka 5, Fukuoka 6, and Fukuoka 7) by Western blotting.
