TABLE 2.
Purpose | Gene | Primer | Oligonucleotide sequence (5′→3′) | PCR product (bp) |
---|---|---|---|---|
Cloning | ses | ORF6GSTF | CCCCGGATCCGATGAATCTAGACCTAAAATAG | 794 |
ORF6GSTR | CCCCGTCGACTTATTGGGAATAAAC | |||
set | ORF5GSTF | CCCCGGATCCGATTCTCGTGAAGGTTTAAAAG | 671 | |
ORF5GSTR | CCCCGTCGACCTATTTTTCCATATATATATC | |||
RT-PCR | ses | ORF6F | TTCAGAAATAGCCAATCATTTCAA | 195 |
ORF6R | CCTTTTTGTTGAGAGCCGTC | |||
set | ORF5F | GGTGATTATGTAGATGCTTGGG | 170 | |
ORF5R | TCGGGTGTTACTTCTGTTTGC | |||
femA | femA1 | AAAAAAGCACATAACAAGCG | 134 | |
femA2 | GATAAAGAAGAAACCAGCAG |