Figure 7.

Shear resistance of K562 transfectants on rsVCAM-1 or FN40. Using initial conditions as in Figure 6, A, B, and D, an average of ∼50 cells was allowed to accumulate in shear flow for 15 to 30 s on rsVCAM-1 coated at the indicated levels (A–C). Then cells were subjected to increasing shear stresses in small increments at 10-s intervals. After each 10-s interval, the percentage of cells remaining bound was determined at each shear. In D, cells were allowed to accumulate in static conditions for 2 min on FN40 (coated at 2 μg/ml). Then resistance to detachment was determined as for A–C. The values indicated in the detachment profiles (A, B, and D) are the mean of three to four experiments. Results in C are representative of four different experiments.