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. 1992 Aug;60(8):3468–3471. doi: 10.1128/iai.60.8.3468-3471.1992

Purification and immunological studies of the cross-reaction between the 65-kilodalton gonococcal parietal lectin and an antigen common to a wide range of bacteria.

R Benkirane 1, R Guinet 1, T Delaunay 1
PMCID: PMC257341  PMID: 1639519

Abstract

The 65-kDa gonococcal parietal lectin (GPL) has been purified and found to have a lectinlike activity exhibiting both structural and immunological similarities to the common antigen family. Ultrastructural localization of GPL was done by using anti-GPL monoclonal antibodies: GPL is a major component of the outer membrane and is also present in blebs formed by gonococci.

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Selected References

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  1. Bibb W. F., Arnow P. M., Thacker L., McKinney R. M. Detection of soluble Legionella pneumophila antigens in serum and urine specimens by enzyme-linked immunosorbent assay with monoclonal and polyclonal antibodies. J Clin Microbiol. 1984 Sep;20(3):478–482. doi: 10.1128/jcm.20.3.478-482.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Galfrè G., Milstein C. Preparation of monoclonal antibodies: strategies and procedures. Methods Enzymol. 1981;73(Pt B):3–46. doi: 10.1016/0076-6879(81)73054-4. [DOI] [PubMed] [Google Scholar]
  3. Hindersson P., Petersen C. S., Pedersen N. S., Høiby N., Axelsen N. H. Immunological cross-reaction between antigen Tp-4 of Treponema pallidum and an antigen common to a wide range of bacteria. Acta Pathol Microbiol Immunol Scand B. 1984 Aug;92(4):183–188. doi: 10.1111/j.1699-0463.1984.tb02818.x. [DOI] [PubMed] [Google Scholar]
  4. Hoffman P. S., Houston L., Butler C. A. Legionella pneumophila htpAB heat shock operon: nucleotide sequence and expression of the 60-kilodalton antigen in L. pneumophila-infected HeLa cells. Infect Immun. 1990 Oct;58(10):3380–3387. doi: 10.1128/iai.58.10.3380-3387.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  6. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  7. Perrollet H., Guinet R. M. Gonococcal cell wall lectin-adhesin with vaccine potential. Lancet. 1986 May 31;1(8492):1269–1270. doi: 10.1016/s0140-6736(86)91404-2. [DOI] [PubMed] [Google Scholar]
  8. Plikaytis B. B., Carlone G. M., Pau C. P., Wilkinson H. W. Purified 60-kilodalton Legionella protein antigen with Legionella-specific and nonspecific epitopes. J Clin Microbiol. 1987 Nov;25(11):2080–2084. doi: 10.1128/jcm.25.11.2080-2084.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Robinson E. N., Jr, McGee Z. A., Kaplan J., Hammond M. E., Larson J. K., Buchanan T. M., Schoolnik G. K. Ultrastructural localization of specific gonococcal macromolecules with antibody-gold sphere immunological probes. Infect Immun. 1984 Nov;46(2):361–366. doi: 10.1128/iai.46.2.361-366.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sompolinsky D., Hertz J. B., Høiby N., Jensen K., Mansa B., Pedersen V. B., Samra Z. An antigen common to a wide range of bacteria. 2. A biochemical study of a "common antigen" from Pseudomonas aeruginosa. Acta Pathol Microbiol Scand B. 1980 Oct;88(5):253–260. doi: 10.1111/j.1699-0463.1980.tb02637.x. [DOI] [PubMed] [Google Scholar]
  11. Thole J. E., Hindersson P., de Bruyn J., Cremers F., van der Zee J., de Cock H., Tommassen J., van Eden W., van Embden J. D. Antigenic relatedness of a strongly immunogenic 65 kDA mycobacterial protein antigen with a similarly sized ubiquitous bacterial common antigen. Microb Pathog. 1988 Jan;4(1):71–83. doi: 10.1016/0882-4010(88)90049-6. [DOI] [PubMed] [Google Scholar]
  12. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]

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